Determination of amino acid profile for argininosuccinic aciduria disorder using High-Performance Liquid Chromatography with fluorescence detection
نویسندگان
چکیده
منابع مشابه
Determination of Astaxanthin in Trout by High Performance Liquid Chromatography Coupled with Visible Detection
Background: Astaxanthin is widely and naturally distributed in marine animals including, crustaceans, such as shrimp, crabs, and fish, such as salmon and trout. Methods: A total of 20 trout samples were collected from local market and were analyzed for astaxanthin via HPLC-UV. Analyte extraction was done by water, methanol, dichloromethane, and acetonitrile 4.5:85:5:45.5, v/v/v/v. Results: Desp...
متن کاملSimultaneous determination of furosemide and amiloride in plasma using high-performance liquid chromatography with fluorescence detection.
A high-performance liquid chromatographic method using fluorescence detection for the simultaneous determination of furosemide and amiloride is described. The chromatographic system is based on reversed-phase ion-pair chromatography with sodium dodecylsulphate as ion-pairing agent. The same counter-ion is used for the ion-pair liquid-liquid extraction to ethyl acetate. The minimum detectable co...
متن کاملArgininosuccinic aciduria, an inborn error of amino acid metabolism.
It is probable that many, if not all, of the inherited ('inborn') metabolic disturbances result from a specific enzyme defect, arising from a single abnormal gene. Examples are now known of a number of such inherited defects, some involving amino acids, which result in a block of a metabolic pathway. For example, the normal pathway for the metabolism of phenylalanine and tyrosine is by a series...
متن کاملDetermination of Vitamin C (Ascorbic Acid) Using High Performance Liquid Chromatography Coupled with Electrochemical Detection
Vitamin C (ascorbic acid, ascorbate, AA) is a water soluble organic compound that participates in many biological processes. The main aim of this paper was to utilize two electrochemical detectors (amperometric - Coulouchem III and coulometric - CoulArray) coupled with flow injection analysis for the detection of ascorbic acid. Primarily, we optimized the experimental conditions. The optimized ...
متن کاملdetermination of gamma - aminobutyric acid by high-performance liquid chromatography
gamma-aminobutyric acid is the main inhibitory amino acid neurotransmitter in several areas of the brain. measurement of gamma-aminobutyric acid in the brain will help elucidate its metabolic rote and diagnostic value. the liquid chromatography (lc) system consisted of a delivery pump. male rats (150-250g) were used in this study. the brain was rapidly removed from decapitated animals. trie chr...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Acta Biochimica Polonica
سال: 2020
ISSN: 1734-154X,0001-527X
DOI: 10.18388/abp.2020_5164